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A general protocol for the generation of Nanobodies for structural biology
There is growing interest in using antibodies as auxiliary proteins to crystallize proteins. Here, we describe a general protocol for the generation of Nanobodies to be used as crystallization chaperones for the structural investigation of diverse conformational states of flexible (membrane) protein...
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| 出版年: | Nat Protoc |
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| 主要な著者: | , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2014
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4297639/ https://ncbi.nlm.nih.gov/pubmed/24577359 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.039 |
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