Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

Structural, biochemical and biophysical studies of eukaryotic membrane proteins are often hampered by difficulties in over-expression of the candidate molecule. Baculovirus transduction of mammalian cells (BacMam), although a powerful method to heterologously express membrane proteins, can be cumber...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nat Protoc
Egile Nagusiak: Goehring, April, Lee, Chia-Hsueh, Wang, Kevin H., Michel, Jennifer Carlisle, Claxton, Derek P., Baconguis, Isabelle, Althoff, Thorsten, Fischer, Suzanne, Garcia, K. Christopher, Gouaux, Eric
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2014
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4291175/
https://ncbi.nlm.nih.gov/pubmed/25299155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.173
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