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Screening and large-scale expression of membrane proteins in mammalian cells for structural studies
Structural, biochemical and biophysical studies of eukaryotic membrane proteins are often hampered by difficulties in over-expression of the candidate molecule. Baculovirus transduction of mammalian cells (BacMam), although a powerful method to heterologously express membrane proteins, can be cumber...
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| Publicado no: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4291175/ https://ncbi.nlm.nih.gov/pubmed/25299155 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.173 |
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