Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

Structural, biochemical and biophysical studies of eukaryotic membrane proteins are often hampered by difficulties in over-expression of the candidate molecule. Baculovirus transduction of mammalian cells (BacMam), although a powerful method to heterologously express membrane proteins, can be cumber...

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Bibliographic Details
Published in:Nat Protoc
Main Authors: Goehring, April, Lee, Chia-Hsueh, Wang, Kevin H., Michel, Jennifer Carlisle, Claxton, Derek P., Baconguis, Isabelle, Althoff, Thorsten, Fischer, Suzanne, Garcia, K. Christopher, Gouaux, Eric
Format: Artigo
Language:Inglês
Published: 2014
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4291175/
https://ncbi.nlm.nih.gov/pubmed/25299155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.173
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