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Outward-facing conformers of LacY stabilized by nanobodies

The lactose permease of Escherichia coli (LacY), a highly dynamic polytopic membrane protein, catalyzes stoichiometric galactoside/H(+) symport by an alternating access mechanism and exhibits multiple conformations, the distribution of which is altered by sugar binding. We have developed single-doma...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Smirnova, Irina, Kasho, Vladimir, Jiang, Xiaoxu, Pardon, Els, Steyaert, Jan, Kaback, H. Ronald
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2014
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4284591/
https://ncbi.nlm.nih.gov/pubmed/25512549
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1422265112
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