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Escherichia coli dihydrofolate reductase catalyzed proton and hydride transfers: Temporal order and the roles of Asp27 and Tyr100

The reaction catalyzed by Escherichia coli dihydrofolate reductase (ecDHFR) has become a model for understanding enzyme catalysis, and yet several details of its mechanism are still unresolved. Specifically, the mechanism of the chemical step, the hydride transfer reaction, is not fully resolved. We...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Liu, C. Tony, Francis, Kevin, Layfield, Joshua P., Huang, Xinyi, Hammes-Schiffer, Sharon, Kohen, Amnon, Benkovic, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4280594/
https://ncbi.nlm.nih.gov/pubmed/25453098
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1415940111
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