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Nonperfect Synchronization of Reaction Center Rehybridization in the Transition State of the Hydride Transfer Catalyzed by Dihydrofolate Reductase

It has been suggested that the magnitudes of secondary kinetic isotope effects (2° KIEs) of enzyme-catalyzed reactions are an indicator of the extent of reaction-center rehybridization at the transition state. A 2° KIE value close to the corresponding secondary equilibrium isotope effects (2° EIE) i...

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Dettagli Bibliografici
Pubblicato in:J Am Chem Soc
Autori principali: Pu, Jingzhi, Ma, Shuhua, Garcia-Viloca, Mireia, Gao, Jiali, Truhlar, Donald G., Kohen, Amnon
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4477101/
https://ncbi.nlm.nih.gov/pubmed/16231943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja054170t
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