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A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine
Mouse embryonic stem cells (ESCs) can be transfected by electroporation, liposomal reagents, and viral transduction methods. The cationic polymer polyethylenimine (PEI) has been shown to transfect a variety of differentiated mammalian cell types, including mouse ESCs, but existing methods require th...
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| Udgivet i: | Exp Cell Res |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4268182/ https://ncbi.nlm.nih.gov/pubmed/25102378 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.yexcr.2014.07.020 |
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