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A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine
Mouse embryonic stem cells (ESCs) can be transfected by electroporation, liposomal reagents, and viral transduction methods. The cationic polymer polyethylenimine (PEI) has been shown to transfect a variety of differentiated mammalian cell types, including mouse ESCs, but existing methods require th...
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| Publicado no: | Exp Cell Res |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4268182/ https://ncbi.nlm.nih.gov/pubmed/25102378 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.yexcr.2014.07.020 |
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