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Fast and Efficient Transfection of Mouse Embryonic Stem Cells Using Non-Viral Reagents

Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. We have found that transfecti...

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Detalhes bibliográficos
Publicado no:Stem Cell Rev
Main Authors: Tamm, Christoffer, Kadekar, Sandeep, Pijuan-Galitó, Sara, Annerén, Cecilia
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5050252/
https://ncbi.nlm.nih.gov/pubmed/27358240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12015-016-9673-5
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