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Membrane translocation assay based on proteolytic cleavage: Application to diphtheria toxin T domain

The function of diphtheria toxin translocation (T) domain is to transfer the catalytic domain across the endosomal membrane upon acidification. The goal of this study was to develop and apply an in vitro functional assay for T domain activity, suitable for investigation of structure-function relatio...

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Detalles Bibliográficos
Publicado en:Biochim Biophys Acta
Main Authors: Rodnin, Mykola V., Ladokhin, Alexey S.
Formato: Artigo
Idioma:Inglês
Publicado: 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4259893/
https://ncbi.nlm.nih.gov/pubmed/25291602
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbamem.2014.09.013
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