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Membrane translocation assay based on proteolytic cleavage: Application to diphtheria toxin T domain

The function of diphtheria toxin translocation (T) domain is to transfer the catalytic domain across the endosomal membrane upon acidification. The goal of this study was to develop and apply an in vitro functional assay for T domain activity, suitable for investigation of structure-function relatio...

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Publicat a:Biochim Biophys Acta
Autors principals: Rodnin, Mykola V., Ladokhin, Alexey S.
Format: Artigo
Idioma:Inglês
Publicat: 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4259893/
https://ncbi.nlm.nih.gov/pubmed/25291602
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbamem.2014.09.013
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