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KINETIC INTERMEDIATE REVEALS STAGGERED PH-DEPENDENT TRANSITIONS ALONG THE MEMBRANE INSERTION PATHWAY OF DIPHTHERIA TOXIN T-DOMAIN

The pH-triggered membrane insertion pathway of the T-domain of diphtheria toxin was studied using site-selective fluorescence labeling with subsequent application of several spectroscopic techniques (e.g., fluorescence correlation spectroscopy, FRET, lifetime quenching and kinetic fluorescence). FCS...

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Detalhes bibliográficos
Main Authors: Kyrychenko, Alexander, Posokhov, Yevgen O., Rodnin, Mykola V., Ladokhin, Alexey S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2748921/
https://ncbi.nlm.nih.gov/pubmed/19588969
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi9009264
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