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High throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry
Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human le...
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| Publicat a: | Nat Commun |
|---|---|
| Autors principals: | , , , , , , , , , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4256720/ https://ncbi.nlm.nih.gov/pubmed/25472703 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms6641 |
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