High throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry

Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human le...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:Nat Commun
Autors principals: Porichis, Filippos, Hart, Meghan G., Griesbeck, Morgane, Everett, Holly L., Hassan, Muska, Baxter, Amy E., Lindqvist, Madelene, Miller, Sara M., Soghoian, Damien Z., Kavanagh, Daniel G., Reynolds, Susan, Norris, Brett, Mordecai, Scott K., Nguyen, Quan, Lai, Chunfai, Kaufmann, Daniel E.
Format: Artigo
Idioma:Inglês
Publicat: 2014
Matèries:
Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4256720/
https://ncbi.nlm.nih.gov/pubmed/25472703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms6641
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars