High throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry

Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single cell level. Here we describe optimized protocols that exploit a highly sensitive FISH method based on branched DNA technology to detect mRNA and miRNA in human le...

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Vydáno v:Nat Commun
Hlavní autoři: Porichis, Filippos, Hart, Meghan G., Griesbeck, Morgane, Everett, Holly L., Hassan, Muska, Baxter, Amy E., Lindqvist, Madelene, Miller, Sara M., Soghoian, Damien Z., Kavanagh, Daniel G., Reynolds, Susan, Norris, Brett, Mordecai, Scott K., Nguyen, Quan, Lai, Chunfai, Kaufmann, Daniel E.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4256720/
https://ncbi.nlm.nih.gov/pubmed/25472703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms6641
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