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Miniaturized High-Throughput Fluorescent Assay for Conversion of NAD(P)H to NAD(P)
A miniaturized fluorescent assay is described that monitors the conversion of NADPH to NADP(+). The same assay format may also be used to measure NADH to NAD(+) conversion. Examples of assay development and validation results are presented to illustrate the use of this protocol to screen an enzyme t...
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| 發表在: | Curr Protoc Chem Biol |
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| Main Authors: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2011
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4250932/ https://ncbi.nlm.nih.gov/pubmed/25478291 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470559277.ch100155 |
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