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High-throughput fluorescence assay of cytochrome P450 3A4
Microtiter plate-based fluorescence assays allow rapid measurement of the catalytic activities of cytochrome P450 oxygenases (P450s). We describe a high-throughput fluorescence assay of P450 3A4, one of the key enzymes involved in xenobiotic metabolism. The assay involves the oxidative debenzylation...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2013
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4024206/ https://ncbi.nlm.nih.gov/pubmed/23475676 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-321-3_14 |
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