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High-throughput fluorescence assay of cytochrome P450 3A4
Cytochrome P450 mono-oxygenases (P450s) are the principal enzymes involved in the oxidative metabolism of drugs and other xenobiotics. In this protocol, we describe a fluorescence-based, high-throughput assay for measuring the activity of P450 3A4, one of the key enzymes involved in drug metabolism....
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3843962/ https://ncbi.nlm.nih.gov/pubmed/19661996 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.123 |
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