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High-throughput fluorescence assay of cytochrome P450 3A4

Microtiter plate-based fluorescence assays allow rapid measurement of the catalytic activities of cytochrome P450 oxygenases (P450s). We describe a high-throughput fluorescence assay of P450 3A4, one of the key enzymes involved in xenobiotic metabolism. The assay involves the oxidative debenzylation...

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Detalhes bibliográficos
Main Authors: Cheng, Qian, Guengerich, F. Peter.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4024206/
https://ncbi.nlm.nih.gov/pubmed/23475676
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-321-3_14
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