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A Novel High-Throughput Cell-Based Assay Aimed at Identifying Inhibitors of DNA Metabolism in Bacteria

Bacterial biosensor strains can be useful tools for the discovery and characterization of antibacterial compounds. A plasmid-based reporter vector containing a transcriptional fusion between the recA promoter and green fluorescence protein gene was introduced into an Escherichia coli ΔtolC strain to...

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Detalhes bibliográficos
Publicado no:Antimicrob Agents Chemother
Main Authors: Fan, Jun, de Jonge, Boudewijn L. M., MacCormack, Kathy, Sriram, Shubha, McLaughlin, Robert E., Plant, Helen, Preston, Marian, Fleming, Paul R., Albert, Robert, Foulk, Melinda, Mills, Scott D.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4249574/
https://ncbi.nlm.nih.gov/pubmed/25246396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.03475-14
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