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Use of shRNA for Stable Suppression of Chemokine Receptor Expression and Function in Human Cancer Cell Lines

In this chapter, we describe a protocol used for stable silencing of chemokine receptor CXCR7 in human cancer cells using shRNA in a lipid transfection setting, previously published by our laboratory. We provide thorough detail and background information about the process of shRNA to clarify the imp...

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Publicado en:Methods Mol Biol
Autores principales: Salazar, Nicole, Muñoz, Daniel, Hoy, James, Lokeshwar, Bal L.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4236910/
https://ncbi.nlm.nih.gov/pubmed/24908308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-0928-5_19
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