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Use of shRNA for Stable Suppression of Chemokine Receptor Expression and Function in Human Cancer Cell Lines
In this chapter, we describe a protocol used for stable silencing of chemokine receptor CXCR7 in human cancer cells using shRNA in a lipid transfection setting, previously published by our laboratory. We provide thorough detail and background information about the process of shRNA to clarify the imp...
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| Udgivet i: | Methods Mol Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4236910/ https://ncbi.nlm.nih.gov/pubmed/24908308 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-0928-5_19 |
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