Expression, purification and characterization of soluble red rooster laforin as a fusion protein in Escherichia coli

BACKGROUND: The gene that encodes laforin, a dual-specificity phosphatase with a carbohydrate-binding module, is mutated in Lafora disease (LD). LD is an autosomal recessive, fatal progressive myoclonus epilepsy characterized by the intracellular buildup of insoluble, hyperphosphorylated glycogen-li...

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Udgivet i:BMC Biochem
Main Authors: Brewer, M Kathryn, Husodo, Satrio, Dukhande, Vikas V, Johnson, Mary Beth, Gentry, Matthew S
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4234410/
https://ncbi.nlm.nih.gov/pubmed/24690255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-15-8
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