Overproduction, crystallization and preliminary X-ray crystallographic analysis of Escherichia coli tRNA N (6)-threonylcarbamoyladenosine dehydratase

Escherichia coli tRNA N (6)-threonylcarbamoyladenosine dehydratase (TcdA), previously called CsdL or YgdL, was overproduced and purified from E. coli and crystallized using polyethylene glycol 3350 as a crystallizing agent. X-ray diffraction data were collected to 2.70 Å resolution under cryoconditi...

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Publicado en:Acta Crystallogr F Struct Biol Commun
Autores principales: Kim, Sunmin, Kim, Keon Young, Park, Jeong Kuk, Lee, Byung Il, Kim, Yun-Gon, Park, SangYoun
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2014
Materias:
Crystallization Communications
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4231855/
https://ncbi.nlm.nih.gov/pubmed/25372820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14020883
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