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Overproduction, crystallization and preliminary X-ray crystallographic analysis of Escherichia coli tRNA N (6)-threonylcarbamoyladenosine dehydratase
Escherichia coli tRNA N (6)-threonylcarbamoyladenosine dehydratase (TcdA), previously called CsdL or YgdL, was overproduced and purified from E. coli and crystallized using polyethylene glycol 3350 as a crystallizing agent. X-ray diffraction data were collected to 2.70 Å resolution under cryoconditi...
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| Publicado no: | Acta Crystallogr F Struct Biol Commun |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4231855/ https://ncbi.nlm.nih.gov/pubmed/25372820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14020883 |
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