Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron

Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10 Å resolution at 100 K using synchrotron X-rays. The crys...

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Detalhes bibliográficos
Main Authors: Cho, Jea-Won, Han, Byeong-Gu, Park, Sang Youn, Kim, Seung Jun, Kim, Myoung-Dong, Lee, Byung Il
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3792672/
https://ncbi.nlm.nih.gov/pubmed/24100564
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113023877
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