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Crystallization and preliminary X-ray crystallographic analysis of carboxyl-terminal region 4 of SigR from Streptomyces coelicolor A3(2)

Full-length SigR from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli, purified and submitted to crystallization trials using either polyethylene glycol 3350 or 4000 as a precipitant. X-ray diffraction data were collected to 2.60 Å resolution under cryoconditions using synchrotro...

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Detalhes bibliográficos
Main Authors: Kim, Keon Young, Kim, Sunmin, Park, Jeong Kuk, Song, HyoJin, Park, SangYoun
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4051528/
https://ncbi.nlm.nih.gov/pubmed/24915084
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14008437
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