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A well-differentiated B-cell line is permissive for somatic mutation of a transfected immunoglobulin heavy-chain gene.

pSV2neo plasmids containing an IgM heavy-chain gene with nonsense mutations in either the variable (V) or the constant (C) region were transfected into four differentiated mouse plasma cell lines: S107 and the NSO fusion partner (myeloma cell lines) and 2C3 and 36.65 (hybridoma cell lines). The freq...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Zhu, M, Rabinowitz, J L, Green, N S, Kobrin, B J, Scharff, M D
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1995
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42308/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7708729/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.7.2810
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