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Simulate_PCR for amplicon prediction and annotation from multiplex, degenerate primers and probes

BACKGROUND: Pairing up primers to amplify desired targets and avoid undesired cross reactions can be a combinatorial challenge. Effective prediction of specificity and inclusivity from multiplexed primers and TaqMan®/Luminex® probes is a critical step in PCR design. RESULTS: Code is described to ide...

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Publicat a:BMC Bioinformatics
Autors principals: Gardner, Shea N, Slezak, Tom
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4226945/
https://ncbi.nlm.nih.gov/pubmed/25005023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-15-237
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