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Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes

Background. Targeted enrichment improves coverage of highly mutable viruses at low concentration in complex samples. Degenerate primers that anneal to conserved regions can facilitate amplification of divergent, low concentration variants, even when the strain present is unknown. Results. A tool for...

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Bibliografische gegevens
Hoofdauteurs: Gardner, Shea N., Jaing, Crystal J., Elsheikh, Maher M., Peña, José, Hysom, David A., Borucki, Monica K.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Hindawi Publishing Corporation 2014
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4137498/
https://ncbi.nlm.nih.gov/pubmed/25157264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/101894
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