Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes

Background. Targeted enrichment improves coverage of highly mutable viruses at low concentration in complex samples. Degenerate primers that anneal to conserved regions can facilitate amplification of divergent, low concentration variants, even when the strain present is unknown. Results. A tool for...

Full description

Saved in:
Bibliographic Details
Main Authors: Gardner, Shea N., Jaing, Crystal J., Elsheikh, Maher M., Peña, José, Hysom, David A., Borucki, Monica K.
Format: Artigo
Language:Inglês
Published: Hindawi Publishing Corporation 2014
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4137498/
https://ncbi.nlm.nih.gov/pubmed/25157264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/101894
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items