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Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes
Background. Targeted enrichment improves coverage of highly mutable viruses at low concentration in complex samples. Degenerate primers that anneal to conserved regions can facilitate amplification of divergent, low concentration variants, even when the strain present is unknown. Results. A tool for...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Hindawi Publishing Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4137498/ https://ncbi.nlm.nih.gov/pubmed/25157264 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/101894 |
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