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Simulate_PCR for amplicon prediction and annotation from multiplex, degenerate primers and probes
BACKGROUND: Pairing up primers to amplify desired targets and avoid undesired cross reactions can be a combinatorial challenge. Effective prediction of specificity and inclusivity from multiplexed primers and TaqMan®/Luminex® probes is a critical step in PCR design. RESULTS: Code is described to ide...
Gespeichert in:
| Veröffentlicht in: | BMC Bioinformatics |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4226945/ https://ncbi.nlm.nih.gov/pubmed/25005023 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-15-237 |
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