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A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy

Tandem fluorescent protein-tagged LC3s that were comprised of a protein tag that emits green fluorescence (e.g., EGFP or mWasabi) fused with another tag that emits red fluorescence (e.g. mCherry or TagRFP) were used for monitoring the maturation step of mammalian autophagosomes. A critical point for...

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Detalhes bibliográficos
Main Authors: Tanida, Isei, Ueno, Takashi, Uchiyama, Yasuo
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4207750/
https://ncbi.nlm.nih.gov/pubmed/25340751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0110600
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