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A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy

Tandem fluorescent protein-tagged LC3s that were comprised of a protein tag that emits green fluorescence (e.g., EGFP or mWasabi) fused with another tag that emits red fluorescence (e.g. mCherry or TagRFP) were used for monitoring the maturation step of mammalian autophagosomes. A critical point for...

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Détails bibliographiques
Auteurs principaux: Tanida, Isei, Ueno, Takashi, Uchiyama, Yasuo
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4207750/
https://ncbi.nlm.nih.gov/pubmed/25340751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0110600
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