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Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Understanding membrane protein trafficking, assembly, and expression requires an approach that differentiates between those residing in intracellular organelles and those localized on the plasma membrane. Traditional fluorescence-based measurements lack the capability to distinguish membrane protein...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Fox-Loe, Ashley M., Henderson, Brandon J., Richards, Christopher I.
Formato: Artigo
Idioma:Inglês
Publicado: MyJove Corporation 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409030/
https://ncbi.nlm.nih.gov/pubmed/28362418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55466
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