In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries.

A method for isolating and cloning mRNA populations from individual cells in living, intact plant tissues is described. The contents of individual cells were aspirated into micropipette tips filled with RNA extraction buffer. The mRNA from these cells was purified by binding to oligo(dT)-linked magn...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Karrer, E E, Lincoln, J E, Hogenhout, S, Bennett, A B, Bostock, R M, Martineau, B, Lucas, W J, Gilchrist, D G, Alexander, D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42052/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7731989/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.9.3814
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!