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A three-fluorophore FRET assay for high-throughput screening of small-molecule inhibitors of ribosome assembly
In one of the first steps of prokaryotic ribosome assembly, the ribosomal protein S15 binds to a three-way junction in the central domain of the 16S rRNA. Binding causes a conformational change that is required for subsequent binding events. Using a novel fluorescence resonance energy transfer assay...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC419595/ https://ncbi.nlm.nih.gov/pubmed/15148358 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh588 |
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