Llwytho...

Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules

We describe an 8-spot confocal setup for high-throughput smFRET assays and illustrate its performance with two characteristic experiments. First, measurements on a series of freely diffusing doubly-labeled dsDNA samples allow us to demonstrate that data acquired in multiple spots in parallel can be...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:PLoS One
Prif Awduron: Ingargiola, Antonino, Lerner, Eitan, Chung, SangYoon, Panzeri, Francesco, Gulinatti, Angelo, Rech, Ivan, Ghioni, Massimo, Weiss, Shimon, Michalet, Xavier
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5395192/
https://ncbi.nlm.nih.gov/pubmed/28419142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0175766
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!