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Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules
We describe an 8-spot confocal setup for high-throughput smFRET assays and illustrate its performance with two characteristic experiments. First, measurements on a series of freely diffusing doubly-labeled dsDNA samples allow us to demonstrate that data acquired in multiple spots in parallel can be...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5395192/ https://ncbi.nlm.nih.gov/pubmed/28419142 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0175766 |
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