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Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
The CRISPR-associated protein Cas9 is an RNA-guided endonuclease that cleaves double-stranded DNAs bearing sequences complementary to a 20-nucleotide segment in the guide RNA(1,2). Cas9 has emerged as a versatile molecular tool for genome editing and gene expression control(3). RNA-guided DNA recogn...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4176945/ https://ncbi.nlm.nih.gov/pubmed/25079318 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature13579 |
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