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Plasmid-based shRNA lentiviral particle production for RNAi applications

Lentiviral vectors have become mainstream gene transfer vehicles for their ability to delivery and integrate into host cells. In RNA interference (RNAi) applications, lentiviral constructs constitutively express dsRNA molecules usually as short hairpin RNA (shRNA) enabling long-term gene silencing a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Shum, David, Djaballah, Hakim
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4170048/
https://ncbi.nlm.nih.gov/pubmed/24939963
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057114539390
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