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Plasmid-based shRNA lentiviral particle production for RNAi applications

Lentiviral vectors have become mainstream gene transfer vehicles for their ability to delivery and integrate into host cells. In RNA interference (RNAi) applications, lentiviral constructs constitutively express dsRNA molecules usually as short hairpin RNA (shRNA) enabling long-term gene silencing a...

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Detalhes bibliográficos
Main Authors: Shum, David, Djaballah, Hakim
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4170048/
https://ncbi.nlm.nih.gov/pubmed/24939963
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057114539390
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