Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency

The analysis of quantitative PCR data usually does not take into account the fact that the increase in fluorescence depends on the monitoring chemistry, the input of ds-DNA or ss-cDNA, and the directionality of the targeting of probes or primers. The monitoring chemistries currently available can be...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Ruijter, Jan M., Lorenz, Peter, Tuomi, Jari M., Hecker, Michael, van den Hoff, Maurice J. B.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Springer Vienna 2014
Gaiak:
Original Paper
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4167442/
https://ncbi.nlm.nih.gov/pubmed/25253910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00604-013-1155-8
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