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Removal of between-run variation in a multi-plate qPCR experiment

Quantitative PCR (qPCR) is the method of choice in gene expression analysis. However, the number of groups or treatments, target genes and technical replicates quickly exceeds the capacity of a single run on a qPCR machine and the measurements have to be spread over more than 1 plate. Such multi-pla...

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Detalles Bibliográficos
Publicado en:Biomol Detect Quantif
Main Authors: Ruijter, Jan M., Ruiz Villalba, Adrián, Hellemans, Jan, Untergasser, Andreas, van den Hoff, Maurice J.B.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4822202/
https://ncbi.nlm.nih.gov/pubmed/27077038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2015.07.001
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