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Removal of between-run variation in a multi-plate qPCR experiment

Quantitative PCR (qPCR) is the method of choice in gene expression analysis. However, the number of groups or treatments, target genes and technical replicates quickly exceeds the capacity of a single run on a qPCR machine and the measurements have to be spread over more than 1 plate. Such multi-pla...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Biomol Detect Quantif
Hauptverfasser: Ruijter, Jan M., Ruiz Villalba, Adrián, Hellemans, Jan, Untergasser, Andreas, van den Hoff, Maurice J.B.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4822202/
https://ncbi.nlm.nih.gov/pubmed/27077038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2015.07.001
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