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Quantification of nascent transcription by bromouridine immunocapture nuclear run-on RT-qPCR
Nuclear run-on (NRO) is a method that measures transcriptional activity via the quantification of biochemically labelled nascent RNA molecules derived from nuclear isolates. Widespread usage of this technique has been limited due to its technical difficulty relative to steady-state total mRNA analys...
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| Pubblicato in: | Nat Protoc |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4790731/ https://ncbi.nlm.nih.gov/pubmed/26182239 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.076 |
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