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Quantification of nascent transcription by bromouridine immunocapture nuclear run-on RT-qPCR
Nuclear run-on (NRO) is a method that measures transcriptional activity via the quantification of biochemically labelled nascent RNA molecules derived from nuclear isolates. Widespread usage of this technique has been limited due to its technical difficulty relative to steady-state total mRNA analys...
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| Publié dans: | Nat Protoc |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4790731/ https://ncbi.nlm.nih.gov/pubmed/26182239 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.076 |
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