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A reliable method for quantification of splice variants using RT-qPCR

BACKGROUND: The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or...

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Bibliografiske detaljer
Udgivet i:BMC Mol Biol
Main Authors: Camacho Londoño, Julia, Philipp, Stephan E.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4793508/
https://ncbi.nlm.nih.gov/pubmed/26979160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12867-016-0060-1
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