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A reliable method for quantification of splice variants using RT-qPCR

BACKGROUND: The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or...

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Publicado en:BMC Mol Biol
Main Authors: Camacho Londoño, Julia, Philipp, Stephan E.
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4793508/
https://ncbi.nlm.nih.gov/pubmed/26979160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12867-016-0060-1
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