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In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes
In this chapter, we describe a method for purification and analysis of the enzymatic activity of deadenylase enzymes. Nearly all eukaryotic messenger RNAs are modified at the 3’ end by addition of an adenosine polymer: the poly-adenosine tail. The poly(A) tail plays a central role in protein express...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4167416/ https://ncbi.nlm.nih.gov/pubmed/24590800 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-971-0_26 |
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