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Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing

Genome sequencing studies have shown that human malignancies often bear mutations in four or more driver genes(1), but it is difficult to recapitulate this degree of genetic complexity in mouse models using conventional breeding. Here we use the CRISPR-Cas9 system of genome editing(2–4) to overcome...

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Detalhes bibliográficos
Main Authors: Heckl, Dirk, Kowalczyk, Monika S., Yudovich, David, Belizaire, Roger, Puram, Rishi V., McConkey, Marie E., Thielke, Anne, Aster, Jon C., Regev, Aviv, Ebert, Benjamin L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4160386/
https://ncbi.nlm.nih.gov/pubmed/24952903
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2951
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