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Measurement of peptide binding to MHC class II molecules by fluorescence polarization
Peptide binding to major histocompatibility complex class II (MHCII) molecules is a key process in antigen presentation and CD4+ T cell epitope selection. This unit describes a fairly simple but powerful fluorescence polarization-based binding competition assay to measure peptide binding to soluble...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4151172/ https://ncbi.nlm.nih.gov/pubmed/25081912 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142735.im0510s106 |
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