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Measurement of peptide binding to MHC class II molecules by fluorescence polarization

Peptide binding to major histocompatibility complex class II (MHCII) molecules is a key process in antigen presentation and CD4+ T cell epitope selection. This unit describes a fairly simple but powerful fluorescence polarization-based binding competition assay to measure peptide binding to soluble...

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Autors principals: Yin, Liusong, Stern, Lawrence J.
Format: Artigo
Idioma:Inglês
Publicat: 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4151172/
https://ncbi.nlm.nih.gov/pubmed/25081912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142735.im0510s106
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