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Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis
Current laboratory methods to isolate rare (1:10,000 to 1:100,000) bacterial artificial chromosome (BAC) recombinants require selectable markers. Seamless BAC mutagenesis needs two steps: isolation of rare recombinants using selectable markers, followed by marker removal through counterselection. He...
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| Autors principals: | , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4149595/ https://ncbi.nlm.nih.gov/pubmed/25028895 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3030 |
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