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Improved seamless mutagenesis by recombineering using ccdB for counterselection
Recombineering, which is the use of homologous recombination for DNA engineering in Escherichia coli, usually uses antibiotic selection to identify the intended recombinant. When combined in a second step with counterselection using a small molecule toxin, seamless products can be obtained. Here, we...
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| Autori principali: | , , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2014
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3950717/ https://ncbi.nlm.nih.gov/pubmed/24369425 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt1339 |
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