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Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2)

Linear plus linear homologous recombination-mediated recombineering (LLHR) is ideal for obtaining natural product biosynthetic gene clusters from pre-digested bacterial genomic DNA in one or two steps of recombineering. The natural product salinomycin has a potent and selective activity against canc...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Yin, Jia, Hoffmann, Michael, Bian, Xiaoying, Tu, Qiang, Yan, Fu, Xia, Liqiu, Ding, Xuezhi, Francis Stewart, A., Müller, Rolf, Fu, Jun, Zhang, Youming
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4602208/
https://ncbi.nlm.nih.gov/pubmed/26459865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep15081
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